›› 2012, Vol. 24 ›› Issue (4): 253-255,.doi: 10.3969/j.issn.1004-616x.2012.04.003

• 论著 • 上一篇    下一篇

丙酮酸乙酯对氯气染毒大鼠肺氧化应激损伤的保护作用

杨东海;张 涛;刘 瑞;刘江正;秦绪军;王 钊;张 伟;柏 桦;海春旭;陈宏莉2   

  1. ( 1. 第四军医大学口腔医学系十二队，陕西 西安 710032；2. 第四军医大学军事预防医学系毒理学教研室，陕西 西安 710032)
  • 收稿日期:2011-11-23 修回日期:2012-03-30 出版日期:2012-07-30 发布日期:2012-07-30
  • 通讯作者: 陈宏莉

Ethyl pyruvate protects against chlorine gas -induced lung oxidative stress

YANG Dong-hai1;ZHANG Tao;LIU Rui;LIU Jiang-zheng;QIN Xu-jun;WANG Zhao;ZHANG Wei;BAI Hua1;HAI Chun-xu;CHEN Hong-li   

  1. ( 1. Squadron 12, Department of Stomatology, Fourth Military Medical University, Xi'an 710032; 2. Department of Toxicology, Fourth Military Medical University, Xi'an 710032, Shaanxi, China)
  • Received:2011-11-23 Revised:2012-03-30 Online:2012-07-30 Published:2012-07-30
  • Contact: CHEN Hong-li

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摘要： 目的： 研究丙酮酸乙酯 (ethyl pyruvate，EP)对于氯气导致的急性肺损伤 (acute lung injury，ALI)和肺组织氧化应激的作用及其机制。方法：30只SD大鼠随机分为阴性对照组、阳性对照组和EP处理组，每组各10只，阴性对照组以空气为对照。阳性对照组和EP处理组给予2 536 mg/m3的氯气动态染毒，染毒时间为20 min，染毒后立即给予生理盐水或EP (40 mg/kg)处理。染毒6 h 后采集样本检测肺组织湿干比、血气、肺组织硫代巴比妥酸反应产物 (thiobarbituric acid reactive substances，TBARs)、氧化型谷胱甘肽 (glutathione disulfide，GSSG)和还原型谷胱甘肽 (glutathione，GSH)含量以及谷胱甘肽过氧化物酶 (glutathione peroxidase，GSH-PX)、谷胱甘肽还原酶 (glutathione reductase，GR)、总超氧化物歧化酶 (total superoxide dismutase，T-SOD)和过氧化氢酶 (catalase，CAT)活性。结果：与阴性对照组比较，氯气中毒后肺组织湿干比显著增加，动脉血氧合指数显著降低，肺组织脂质过氧化产物TBARs、GSSG显著增加，GSH含量以及GSH-PX、GR、T-SOD和CAT活性显著降低 (P均<0.05)。与阳性对照组比较，EP治疗显著改善了以上指标 (P均<0.05)。结论：氯气中毒后引起肺组织氧化应激，造成ALI，EP治疗可以有效减轻氧化应激，缓解急性肺损伤。

关键词: 氯气, 急性肺损伤, 氧化应激, 丙酮酸乙酯

Abstract: OBJECTIVE: To study the protective effect and mechanism of ethyl pyruvate (EP) on acute lung injury (ALI) and oxidative stress induced by chlorine gas. METHODS：Adult male SD rats (200±20 g，about 8 weeks of age) were exposed to chlorine gas of 2536 mg/m3 or normal air for 20 min in a whole-body dynamic exposure chamber. EP (40 mg/kg) or vehicle (saline) was given by intraperitoneal injections immediately after chlorine gas exposure. Rats were killed at 6 hours after chlorine gas exposure，and the wet：dry weight ratio，PaO2/FiO2 ratio，the concentration of thiobarbituric acid reactive substances (TBARs)，glutathione disulfide (GSSG)，glutathione (GSH) and the activity of glutathione peroxidase (GSH-PX)，glutathione reductase (GR)，total superoxide dismutase (T-SOD)，catalase (CAT) in the lung were measured. RESULTS：After chlorine gas exposure，the wet：dry weight ratio，TBARs and GSSG in the lung increased significantly (P<0.05). However，PaO2/FiO2 ratio，GSH and the activities of GSH-PX，GR，T-SOD and CAT deceased (P<0.05). EP treatment attenuated these changes (P<0.05). CONCLUSION：EP treatment attenuated ALI and oxidative stress in the lung induced by chlorine gas.

Key words: chlorine gas, ethyl pyruvate, acute lung injury, oxidative stress

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